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Peptide aptamers are small stable proteins with a high-affinity binding surface for a specific protein. Affimers are an important alternative to antibodies and. Aptamers are synthetic molecules that can be raised against any kind of target, including toxic or non immunogenic ones. They bind their target with affinity. Aptamers are oligonucleotide molecules, selected from large libraries to bind a specific target. ; Isolation process, In vivo, In vitro, In vitro ; Scaffold, mAbs.

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While affinity to proteins ranges from nanomolar to picomolar, aptamers against small molecules tend to have an affinity in the micromolar range (Kd ~ μM). Both. Aptamers are different from antibodies in that they are physically much smaller (15 kDa vs. kDa), are generated in vitro, and can be readily modified. The manner in which aptamers bind to target proteins is different from that of antibodies. Although both aptamer therapeutics and antibody drugs are molecular-.

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The manner in which aptamers bind to target proteins is different from that of antibodies. Although both aptamer therapeutics and antibody drugs are molecular-. Aptamers vs. Antibodies ; Stability, Stable in harsh conditions, Potential loss of function in harsh conditions ; Assays, No secondary reagent is needed. While affinity to proteins ranges from nanomolar to picomolar, aptamers against small molecules tend to have an affinity in the micromolar range (Kd ~ μM). Both.